| Assay Method Information | |
| | Aurora A Kinase Assay |
| Description: | Aurora A kinase activity was determined using a radiometric assay to measure the incorporation of gamma-phosphate from [gamma-33P]-ATP into Kemptide. Incorporated radioactivity was counted using Packard TopCount. IC50 values were determined using a sigmoidal dose response equation from Prism GraphPad Software. |
| Affinity data for this assay | |
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