| Assay Method Information | |
| | VEGF-R2 Receptor-Linked Tyrosine Kinase Assay |
| Description: | Compounds were evaluated for their ability to inhibit the kinase activity using an ELISA-based assay. The 96-well microtiter plate is coated with substrate solution. The reaction is initiated by adding recombinant human VEGF-R2 to reaction mixture containing inhibitor compound. After the incubation, the detection antibody, Eu-N1 antiphosphotyrosine (PT66) antibody, was added, and the fluorescence of the resulting solution was measured using the Victor Multilabel Counter. |
| Affinity data for this assay | |
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