| Assay Method Information | |
| | ChEMBL_1853065 (CHEMBL4353689) |
| Description: | Inhibition of N-terminal GST-tagged recombinant human KDM4B (1 to 500 residues) expressed in baculovirus infected Sf9 insect cells assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 20 mins by AlphaScreen assay |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |