| Assay Method Information | |
| | ChEMBL_1711283 (CHEMBL4121332) |
| Description: | Inhibition of recombinant human N-terminal FLAG-tagged PRMT5 (2 to end residues) /human N-terminal His-tagged MEP50 (2 to end residues) expressed in HEK293F cells using biotinylated H4 derived peptide as substrate pretreated for 15 mins followed by substrate and [3H]-SAM addition measured after 60 mins by liquid scintillation counting method |
| Affinity data for this assay | |
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