Assay Method Information

Assay Name:  ChEMBL_1513804 (CHEMBL3611122)
Description:  Inhibition of recombinant human 5-LO expressed in Escherichia coli BL21 using arachidonic acid as substrate assessed as formation of all-trans isomer of LTB4, 5-H(P)ETE preincubated for 5 to 10 mins followed by substrate addition measured after 10 mins by HPLC analysis
Affinity data for this assay
 

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