| Assay Method Information | |
| | Inhibition Assay of Zika virus |
| Description: | Vero E6 cells (BCRC #60476, Bioresource Collection and Research Center, Hsinchu, Taiwan) were treated with one of Compounds 1-9 at a predetermined concentration for 1 hour at 37° C. The cells were adsorbed with Zika virus (ATCC VR-1843, American Type Culture Collection, Manassas, VA, USA) at MOI=0.02 for 49 hours at 37° C. The cells were fixed with 10% formalin, permeabilized with methanol. The cells were stained with anti-flavivirus E antibody (ATCC HB-112, American Type Culture Collection, Manassas, VA, USA) and anti-mouse IgG-FITC (MP Biomedicals, Irvine, CA, USA). The nuclei were counter stained with DAPI (in blue) (Thermo Fisher Scientific, MA, USA). The E protein expression was measured using a high-content image analysis system (Molecular Devices). The cell viability was determined by MTS. Both EC50 and CC50 were cal |
| Affinity data for this assay | |
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