Affinity DataKi: 0.0360nMAssay Description:HEK293 cell lines stably expressing cloned human NR1/NR2B and NR1/NR2A, respectively, were established according to standard previously described met...More data for this Ligand-Target Pair
3D Structure (crystal)Affinity DataKi: 0.240nMAssay Description:The affinity of ligands was determined via radioligand binding techniques using human recombinant receptors expressed in mammalian clonal cells. Deta...More data for this Ligand-Target Pair
Affinity DataKi: 0.5nM ΔG°: -49.3kJ/moleT: 2°CAssay Description:For the membrane binding, NIH-3T3 cells were grown to 70% confluence in 15-cm2 dishes and transfected with 10 ug of receptor plasmid DNA using Polyfe...More data for this Ligand-Target Pair
Affinity DataKi: 0.5nMAssay Description:Inverse agonist activity at 5-HT2A receptor (unknown origin)More data for this Ligand-Target Pair
Affinity DataKi: 0.860nMAssay Description:Displacement of [3H]ketanserin from human 5-HT2A receptor transfected in Jump-In GripTite HEK293 cell membrane measured after 3 hrs incubation by sci...More data for this Ligand-Target Pair
Affinity DataIC50: 1.90nMAssay Description:R-SAT assays were performed as described previously (Weiner et al., 2001), with the following modifications. In brief, NIH-3T3 cells were grown to 80...More data for this Ligand-Target Pair
Affinity DataIC50: 5nMAssay Description:Inverse agonist activity at human prefrontal cortex 5-HT2A receptor assessed as increase in [35S]GTPgammaS binding by liquid scintillation spectromet...More data for this Ligand-Target Pair
Affinity DataIC50: 9nMAssay Description:Antagonist activity at human 5-HT2A receptor expressed in CHO-K1 cells co-expressing Galpha16 assessed as decrease in serotonin calcium mobilization ...More data for this Ligand-Target Pair
Affinity DataIC50: 10nMAssay Description:Antagonist activity at 5HTR2A (unknown origin) assessed as inhibition of 5-HTR2AMore data for this Ligand-Target Pair
Affinity DataIC50: 13nMAssay Description:Inverse agonist activity at human 5-HT2A receptor expressed in cell membrane assessed as increase in [35S]GTPgammaS binding by liquid scintillation s...More data for this Ligand-Target Pair
Affinity DataIC50: 22nMAssay Description:Antagonist activity at 5HT2A receptor (unknown origin) expressed in HEK293 cells assessed as inhibition of serotonin hydrochloride-induced calcium fl...More data for this Ligand-Target Pair
Affinity DataIC50: 27nMAssay Description:Antagonist activity at 5-HT2A receptor (unknown origin) expressed in CHO-K1 cells assessed as reduction in intracellular Ca2+ mobilization incubated ...More data for this Ligand-Target Pair
Affinity DataIC50: 27.3nMAssay Description:CHO-K1/5-HT2A cells in the logarithmic growth phase were seeded into a 384-well plate at a density of 10000 cells per well, and cultured in a 37° C.,...More data for this Ligand-Target Pair
Affinity DataIC50: 50nMAssay Description:Inverse agonist activity at 5-HT2A receptor (unknown origin) expressed in mouse NIH3T3 cells using o-nitrophenyl-D-galacto pyranoside incubated for 1...More data for this Ligand-Target Pair
Affinity DataIC50: 50nMAssay Description:NIH3T3-5HT2AR cells in the logarithmic growth phase were seeded into a white-wall clear-bottom 96-well plate at a density of 1000 cells per well, and...More data for this Ligand-Target Pair
